DNA

Part:BBa_K874040:Design

Designed by: Ernst Bank   Group: iGEM12_Amsterdam   (2012-09-22)

125bp DNA spacer (inc. preceding BamHI site)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains no forbidden or undesirable sites according to the RFC-10 protocol. Also has been designed to contain no sites matching E.Coli native restriction/methylation systems

Source

Synthesized by IDT as part of BBa_K874120 (methylation measurement module)

References